EFFECT OF PLATELET CONCENTRATION AND CALCIUM ON PLASMA CLOT ABSORBANCE

Modepalli Susree, Mohan Anand

Abstract: Clot structure is greatly influenced by a number of factors in vivo, calcium concentration being one of them. A pilot study with 10 healthy individuals was performed to investigate the effect of added calcium, in combination with plasma platelet concentration, on the turbidity of clots. Using a UV-Vis spectrophotometer, absorbance of both platelet-rich and platelet-poor clots was taken at 405 nm, in the presence as well as the absence of added calcium. A 9.93% increase in the maximum absorbance is observed for PRP with added calcium while a 7.68% increase was observed for PPP. Also, PRP clots reported higher absorbance than PPP clots both with (16.48%) and without (14.10%) calcium. It was also observed in parallel that platelet concentration also affected turbidity of the clots with & without added calcium

Keywords: Calcium, Platelet-Poor Plasma (PPP), Platelet-Rich Plasma (PRP), Thrombin, and Turbidity

DOI: https://doi.org/10.15623/ijret.2016.0506020