CHARACTERIZATIONS AND OPTIMIZATION STUDY ON INFLUENCE OF DIFFERENT PARAMETERS ON FABRICATED SPHERICAL NANOPARTICLES FROM STREPTOMYCES SP. GUT 21 (KU500633)

Pramod P Desai, Prabhurajeshwar, B.V.Hudge, Krishnaraj P U, Kelmani Chandrakanth R

Abstract: A new technological approach was used for the production of silver nanoparticles using metal acquiring microorganism as a means of fabrication and making of superior biomaterials. Silver nanoparticles (AgNPs) are nanoparticles of silver which are in the range of 1 and 100 nm in size. Extracellular synthesis of AgNPs was observed when Streptomyces species was exposed to colloidal silver salt solution optimised at 1mM ionic strength by autoclaving method.UV-Visible spectroscopic analysis were done to monitor the drift in change of pH, precursor concentration, Streptomyces supernatant volume and effect of temperature on the formation of AgNPs. Influence of different factors such as pH , initial silver molar strength, water dilution, and methodology used o synthesize nanoparticles were found to be extremely reliable for consistent stability and storage purpose. The results indicated that temperature optimised was at 280C for storage, pH was at neutral 7 and water orchestrated as “diluent”, showed increased Nano-particle spectra. The formation of nanoparticles was first monitored by measuring the surface plasmon resonance (SPR) band at 410nm by UV-Visible absorption spectroscopy. The presence of elemental silver and the crystalline structure of the AgNPs were confirmed by Thermogravimetric analysis (TGA) and Powder X-ray diffraction (PXRD).In addition, Atomic Force Microscopy (AFM) along with FESEM imaging used for characterising nanoparticles revealed that they in spherical shape with an average particle size of 20-70nm.Finally, FTIR investigations revealed that many efficient clusters of functional bio molecules are playing significant role in capping and synthesis process.

Keywords: Antagonism, Silver Nanoparticles (AgNPs), 16S Ribosomal Gene, Atomic Force Microscopy (AFM).

DOI: https://doi.org/10.15623/ijret.2016.0502028