IDENTIFICATION AND CHEMICAL CHARACTERIZATION OF AZADIRACHTA INDICA LEAF EXTRACTS THROUGH THIN LAYER CHROMATOGRAPHY

M. Mukunda Vani, P. Sagar Suresh Rao, G. Nikhilesh Varma, K.Pushpitha, Linisha Biswal

Abstract: In this article, extraction of Azadirachta indica compounds has been done using solvents namely ethanol, methanol, benzene, ethyl acetate, toluene which are widely used in industries as solvents. All the compounds present in Azadirachta indica leaves are supposed to be soluble in solvents but this work has been done to show that different solvents have different capacity to extract the compounds. The semi quantitative analysis has been done with the help of thin layer chromatography (TLC) which shows the efficiency of every individual solvent to extract compounds from Azadirachta indica. Thin layer chromatography can physically separate the compounds from pure extracts hence this method is preferred for analysis purpose. Even composition and their respective percentage can be concluded using various analysis method. It is helpful to analyse the solubility of different compounds of Azadirachta indica in particular solvent.

Keywords: Extraction, Thin Layer Chromatography (TLC), Semi Quantitative Analysis, Solubility Of Compounds, Characterization And Identification

DOI: https://doi.org/10.15623/ijret.2016.0502021