DECOLORIZATION POTENTIAL OF IMMOBILIZED PSEUDOMONAS PUTIDA MTCC 1194 WITH LOW COST ADSORBENT FOR REACTIVE DYE

A.Ullhyan, U.K. Ghosh

Abstract: A simultaneous adsorption and biodegradation method for removal of reactive blue-4 is reported here. The mustard stalk activated carbon (MSAC) using Pseudomonas putida MTCC1194 was applied to decolorize reactive blue-4. Batch studies were performed to evaluate the influences of various parameters; initial pH, adsorbent dose, temperature and initial concentration, on removal of reactive blue-4. Optimum conditions for reactive blue -4 removals were found to be pH 7, adsorbent dose10g/l, temperature 32°C at equilibrium time 360 min for 150 mg/l of dye concentration. Experimental data were analyzed by pseudofirst order, pseudo-second order kinetics and intra-particle diffusion model. Equilibrium isotherms for the adsorption of reactive blue 4 onto MSAC were analyzed by Freundlich, Langmuir, Temkin and Dubinin–Radushkevich (D-R). The results show that experimental data follow pseudo-second order kinetics and intra-particle diffusion model. Out of four isotherms, Langmuir was found to be best fit with experimental data (R2 > 0.97), with 70.2% removal of reactive blue 4.

Keywords: activated carbon mustard stalk, reactive blue 4, simultaneous adsorption and biodegradation (SAB), Pseudomonas putida

DOI: https://doi.org/10.15623/ijret.2015.0409068