ANTIMICROBIAL ACTIVITY OF TRACHYSPERMUM AMMI LEAVES MEDIATED SILVER NANOPARTICLES : GREEN APPROACH

Sonam Sahu, Deepti Rawat, Darshan Singh

Abstract: This paper describes a cost effective and environment friendly technique for green synthesis of silver nanoparticles from 3mM AgNO3 solution through the extract of Trachyspermum ammi leaf using domestic microwave irradiation method. On mixing leaf extract with silver salt solution in ratio 1:4 the color changed from colourless to yellowish brown which partially confirmed the degradation of silver ions to silver nanoparticles (SNPs). The synthesized nanoparticles were characterized by using UV–visible spectroscopic techniques, Energy dispersive X-ray spectroscopy (EDAX), Scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FT-IR). The shape of the synthesized nanoparticles was found to be cubical. The antimicrobial activities of the synthesized silver nanoparticles against two human pathogens were evaluated by using disc diffusion and broth dilution method.

Keywords: Trachyspermum ammi Leaves, SNPs, MIC, SEM-EDAX, Green Synthesis

DOI: https://doi.org/10.15623/ijret.2015.0409006