IMPORTANT DATABASES AND TOOLS TO IDENTIFY PROMISING DRUG TARGETS BY SUBTRACTIVE GENOMICS APPROACH – A REVIEW

Preeti Dora, V.G. Shanmuga Priya, Preeti.S.H, U.M. Muddapur, Rakesh.N.R

Abstract: Most of the pathogenic organisms have antibiotic resistant strains hence there is a need to identify new drug targets to design new drugs to combat the disease.Subtractive genomics approach is one of the recently adopted methodologies to identify novel drug targets specific to the pathogen to avoid any cross reactivity and side effects.This methodology uses various tools and databases to find essential proteins that are indispensible for the survival or virulence of the organism andare absent in the host. A survey was done on the databases and tools generally usedby the researchers to carry out this work on different pathogenic organism. Databases like NCBI (National Centre for Biotechnology Information) genome database, DEG BlastP (Database of Essential Genes and Genomes), BlastP (Basic Local Alignment Search Tool), KEGG (Kyoto Encyclopaedia for Genes and Genomes) database, Drug bank database are used in most of the studies. Tools and web servers such as CD HIT (Cluster database at high identity with tolerance) and CELLO are mostly used.

Keywords: NCBI (National Centre for Biotechnology Information), DEG BlastP (Database of Essential Genes and Genomes), BlastP (Basic Local Alignment Search Tool), KEGG (Kyoto Encyclopaedia for Genes and Genomes), CD HIT(Cluster database at high identity with tolerance), DEG-BlastP ( Database of essential Genes Protein Blast)

DOI: https://doi.org/10.15623/ijret.2015.0406077