BIOSYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES USING FICUS BENGHALENSIS LEAF EXTRACT

Kantrao Saware, Balaji Sawle, Basavraja Salimath, Kamala Jayanthi, Venkataraman Abbaraju

Abstract: The study demonstrates ecofriendly and reproducible synthesis of Silver nanoparticles (SNPs) employing Ficus benghalensis (F.B.) leaf extract). In this paper, we report stable and spherical SNPs of variable size ranging from 10-50 nm. The formation of SNP was monitored using ultraviolet-visible spectroscopy (UV-Vis) technique for surface Plasmon resonance and it is found at 435 nm and absorbance peak at 280 and 240 nm are also found and indicates the protein and hydroxyl anthraquinones capping. The kinetics of effect of time, pH, ionic strength, Concentration of leaf extract and temperature on the formation of SNP was studied using UV-Vis spectroscopy. Fluorescence spectroscopy and Fourier-transform infrared spectroscopy (FTIR) studies indicate the involvement of protein as a capping agent and Quinones as reducing agents. The morphology of the SNPs was determined AFM (Atomic Force Microscopy) and FESEM (Field emission Scanning electron microscopy) along with EDAX. Xray diffraction (XRD) study was carried and found to be face centred cube (fcc).Thermal Gravimetric analysis (TGA) was performed to understand the thermal behaviour of biosynthesized silver nanoparticles. The results indicated that the proteins, which have amine groups, have played a significant role in stabilizing SNPs in the solutions.

Keywords: Ficus benghalensis, Biosynthesis, Silver nanoparticles, Surface Plasmon Resonance, FESEM

DOI: https://doi.org/10.15623/ijret.2014.0305158