BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF ANTAGONISTIC BACTERIA AGAINST YELLOW BLOTCH OF OYSTER MUSHROOM

Anand Kumar, Shiv Kumar Verma, Ashis, Dhiraj Kumar Choudhary

Abstract: Yellow blotch of oyster mushroom (Pleurotus ostreatus) caused by Pseudomonas agarici is considered as a fatal disease to mushroom and diminish food quality for humans. Chemical control of yellow blotch in oyster mushroom has received little efficiency and these chemicals are hazardous to human life, decrease soil fertility and cause pollution. Alternatively biological control is the available strategy against soil borne diseases. The objectives of this research were 1.) To isolate different strains fluorescence, actinomycetes, and phosphate solublizing bacteria in rhizospheric soil from selected plants of different regions. 2.) To examine the antagonistic agent against Pseudomonas agarici. 3.) To characterize the antagonistic bacteria on the basis of biochemical and molecular (16S-r DNA sequencing). 50 strains of fluorescence, actinomycetes, and phosphate solublizing bacteria were collected from different regions. During examination of fluorescence strain K1, K8, K14 were found antagonistic. The above consequences reveal that fluorescence bacteria have high capability of antagonistic property. During biochemical characterization on the basis of methyl-red, VogesProskauer, Indole Production IAA production, gram’s staining, antibiotic assay it was found that antagonistic bacteria showed approximately the same result as Indole production, IAA production gram’s negative and the MIC of Antibiotic assay. Strains were identified Pseudomonas fluorescence, according to the characteristics of its morphology and homology analysis (BLAST) of its 16S-r DNA sequencing.

Keywords: Pseudomonas agarici, Yellow Blotch, Pleurotus ostreatus, 16 S rDNA Sequencing

DOI: https://doi.org/10.15623/ijret.2014.0304052