OPTIMIZATION OF PHYSICAL PARAMETERS OF ?-AMYLASE PRODUCING BREVIBACILLUS BOROSTELENSIS R1 IN SUBMERGED FERMENTATION

K.Suribabu, T. Lalitha Govardhan, K.P.J Hemalatha

Abstract: Bacteria have been regarded as treasure of many useful enzymes viz., amylases, proteases, lipases, hydrolases and reductases. Among them amylolytic enzymes have great biotechnological applications and economic exploitations. The production of a-amylases by fermentation had been thoroughly investigated and shown to be affected by a variety of physicochemical factors, such as the composition of the growth medium, the type of strain, cell growth, methods of cultivation, inoculum concentration, time of incubation, pH, temperature, salinity, carbon, nitrogen and mineral sources. The present study was carried out to optimize the a-amylase production of Brevibacillus borstelensis R1 using ten different media viz., Nutrient broth, Luria Bertain broth, Clarks & Lub medium, Pikovskaya’s medium, Tendlers non-synthetic medium, Amylase production medium, Soluble starch beef extract medium, Soybean casein digest medium, Yeast extract peptone dextrose glucose medium and Tryptone glucose beef extract medium. Among these ten media, Pikovskaya’s (PK) medium proved to be optimal for a-amylase production (1861±17U/ml). The optimized a-amylase production in PK medium by submerged fermentation (SmF) was subjected to varying physical parameters such as 24hrs incubation time, 2% inoculum size, 370C, pH 7.0 and 1% NaCl. Alpha-amylase produced by B.borostelensis R1 have many applications in starch processing, desizing of textiles, paper sizing, detergent additive, bread improvement, ethanol production, sewage treatment, effluent treatment and other fermentation processes.

Keywords: Pikovskaya’s medium, incubation time, inoculum size, temperature, pH and salinity.

DOI: https://doi.org/10.15623/ijret.2014.0301087