PHYTOCHEMICAL ANALYSIS, PROTEIN CONTENT & ANTIMICROBIAL ACTIVITIES OF SELECTED SAMPLES OF GLYCINE MAX LINN

Mamta Arora, Satnam Singh, Ramandeep Kaur

Abstract: Two seed samples of Glycine max Linn. (S1, S2) were purchased from two retail stores of local market. Non-sprouted and sprouted seed powder were extracted separately with methanol (100%, 50%) by cold maceration to obtain methanolic and hydroalcoholic extract of Glycine max Sample 1 was designated as MES1 and HES1 and sample 2 as MES2 and HES2 respectively. Phytochemical analysis indicated the presence of various phytoconstituents viz. phytosterols, flavonoids, phenolic compounds, tannins, carbohydrates, proteins, amino acids, fixed oils and fats etc. Thin layer chromatography study on extracts revealed the presence of a number of compounds. The protein content of these samples were studied. The protein content of samples MES1, HES1, MES2 and HES2 with respect to BSA was found to be 90.6 2µg/ml, 82µg/ml, 94.5µg/ml and 79.1µg/ml respectively. The highest among these were found to be in MES2. Sprouting enhanced the protein content of the two samples. The samples have shown antimicrobial activity at selected concentration and microbial strains (26mm) for gram negative bacteria (27mm) for gram positive bacteria.

Keywords: Glycine max Linn, phytochemical constituents, TLC, antimicrobial activity, protein, methanolic extract, hydroalcoholic extract.

DOI: https://doi.org/10.15623/ijret.2013.0211086